ASTM F / FM – Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using. ASTM F / FM – ASTM International (ASTM). Title. “Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by . STANDARD USED: ASTM F, Standard Test Method for Resistance of Materials Used in Protective. Clothing to Penetration by Blood-Borne Pathogens .

Author: Samunris Goltijind
Country: New Zealand
Language: English (Spanish)
Genre: History
Published (Last): 3 February 2017
Pages: 489
PDF File Size: 14.86 Mb
ePub File Size: 2.64 Mb
ISBN: 865-4-67267-431-7
Downloads: 91086
Price: Free* [*Free Regsitration Required]
Uploader: Tejar

Glove directives and norms list

It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Link to Active This link will always route to sstm current Active version of the standard. The method described in these standards, evaluates the effectiveness of materials used in the manufacture of protective garments against bloodborne pathogens using a surrogate virus suspended in a liquid, simulating the spontaneous contact conditions in a sanitary environment.

This is accomplished by adding surfactant to the Phi-X Bacteriophage nutrient broth.

Please see the full standard for complete details. Examples of test methods include, but are not limited to: The surface tension range for blood and body fluids excluding saliva is approximately 0. If these conditions are of concern, ashm performance of protective clothing materials should be evaluated for Phi-X Bacteriophage penetration following an appropriate preconditioning technique representative of the expected conditions of use.

Clinical Microbiology Rabies Laboratory authorized by the European Union HIV genotypic resistance to antiretrovirals Biocidal activities and toxicology with disinfectants 59 accredited tests Toxicology – Biological evaluation of medical devices; MPCA products 19 accredited tests Cosmetic Microbiology Laboratory of control authorized by AEMPS 8 accredited tests Neutralizing antibodies in human immunoglobulins 3 accredited tests News on viruses: Test Method F uses the same penetration test cell and technique, but exposes material specimens to synthetic blood with visual detection of liquid penetration.

Test not accredited in our laboratory. This test method is based on Test Method F for measuring resistance of chemical protective clothing materials to penetration by liquids.

The method is evaluated by two procedures: This hydrostatic pressure has been documented to discriminate between protective clothing material performance and correlate with visual penetration results that are obtained with a human factors validation 4.


In case a non-sterile material is used, the r1671 will include the corresponding controls to exclude the presence of the bacteriophage in the materials evaluated.

Genetic testing – Human gene mutations diseases, neoplasias and pharmacogenetics Biocidal activities with disinfectants 59 accredited tests Asrm Microbiology Laboratory of control authorized by AEMPS 8 accredited tests Toxicology – Biological aetm of medical devices; Cosmetics; Biocides; MPCA products 19 accredited tests Clostridium botulinum tests Paternity tests in any species, including humans Viruses in water and shellfish Water Microbiology.

To perform the method, samples of the materials are exposed to a nutrient broth containing the bacteriophage virus Phi-X, as a virus surrogated by its characteristics similar in size to the hepatitis C virus, and by extension to the hepatitis B virus and of human immunodeficiency virus these viruses f16771 not used because of asrm difficulty to be cultured in laboratories.

The manufacturer must indicate the possibility that the material under test can be altered by thermal treatment wet sterilizationso as not to do so in the event that it can be altered. Materials passing Test Method F should then be tested against bacteriophage penetration using this test method to verify performance.

Active view current version of standard.

ASTM International – Standard References for ASTM F / FM – 13

Prewetting agents, such as alcohol, and contaminating agents, such as perspiration, may also compromise the integrity of the protective barrier. The visual detection technique of this test method is supplemented with a biologically qstm assay capable of detecting virus under the specified test conditions. Skip directly to search Skip directly to A to Z list Skip directly to navigation Skip directly to page options Skip directly to site content. To protect themselves from infectious agents transmitted by blood, health personnel should wear protective clothing made of materials that prevent microbes or viruses from passing through.

Historical Version s – view previous versions of standard. D Test Method for Retention Characteristics xstm 0. This test method is used to f11671 the resistance of materials used in protective clothing to penetration by blood-borne pathogens using a surrogate microbe under conditions of continuous liquid contact.

Clothing for protection against contact with blood and body fluids — Determination of resistance of protective clothing materials to penetration by blood-borne pathogens – Test method using Phi-X bacteriophage.

Any visual or virological evidence of penetration will indicate the inability of the material to resist penetration. Examples of body protection include laboratory coats, coveralls, vests, jackets, aprons, surgical gowns and full body suits Medical protective clothing.


Inferences for protection from other pathogens must be assessed on a case-by-case basis. The exposure is carried out for a specified time and under a selected pressure. This method is not effective for testing protective clothing materials that are internally coated by a thick coating that can absorb the liquid containing the test virus.

Genetic Testing – Human gene mutations diseases, neoplasias and pharmacogenetics Clostridium botulinum tests Molecular veterinary microbiology Tests for movement of animals South Africa, Australia, India, New Zealand Food microbiology Viruses in water and shellfish Water Microbiology Zebra mussel Dreissena polymorphaother mussels and intermediate hosts molluscs Bulinus spp.

In one of the 60 mL compartments capacity the viral suspension of exposure is introduced, containing the bacteriophage Phi-X and in the opposite compartment the appearance of liquid or the presence of the virus is detected. Because of the length of time required to complete this method, it may not be suitable for use as a material or protective clothing quality control or quality assurance procedure.

November 20, Page last updated: The resulting surface tension of the Phi-X Bacteriophage challenge suspension is approximately 0. This site uses cookies and similar technologies. This method is specifically defined for a penetration model of hepatitis B virus, hepatitis C virus and human immunodeficiency virus, and it is considered by extension that if it resists penetration by these viruses, it will also resist penetration by larger microorganisms like bacteria.

These protective garments can be, for example, gloves, aprons, masks, hoods, or boots, among others.

The test is performed in a chamber of two compartments, separated by the material evaluated. Therefore, it is important to understand that this test method does not simulate all the physical stresses and pressures that might be exerted on protective clothing materials during actual use. The possible alterations of the protective material due to physical, chemical or thermal effects should be taken into account, since in this case they would negatively influence the behavior of the material, so that in case it can occur, the tests should be carried out before exposures to such material, physical, chemical or thermal conditions that may deteriorate it.